Metal trafficking for nitrogen fixation: NifQ donates molybdenum to NifEN/NifH for the biosynthesis of the nitrogenase FeMo-cofactor

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dc.conicet.fuenteindividual
dc.conicet.organizacionUniversity of California
dc.conicet.organizacionConsejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario
dc.conicet.organizacionUniversity of California
dc.conicet.organizacionUniversity of California
dc.conicet.organizacionUniversity of California
dc.conicet.organizacionUniversity of California
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.paisOrganizacionArgentina
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.produccionid6302074
dc.contributor.authorHernandez, Jose A.
dc.contributor.authorCuratti, Leonardo
dc.contributor.authorAznar, Constantino P.
dc.contributor.authorPerova, Zinaida
dc.contributor.authorBritt, R. David
dc.contributor.authorRubio, Luis M.
dc.date.accessioned2021-05-21T15:27:03Z
dc.date.available2021-05-21T15:27:03Z
dc.date.issued2008-08-19
dc.date.updated2021-04-28T21:45:00Z
dc.description.abstractThe molybdenum nitrogenase, present in a diverse group of bacteria and archea, is the major contributor to biological nitrogen fixation. The nitrogenase active site contains an iron-molybdenum cofactor (FeMo-co) composed of 7Fe, 9S, 1Mo, one unidentified light atom, and homocitrate. The nifQ gene was known to be involved in the incorporation of molybdenum into nitrogenase. Here we show direct biochemical evidence for the role of NifQ in FeMo-co biosynthesis. As-isolated NifQ was found to carry a molybdenum-iron-sulfur cluster that serves as a specific molybdenum donor for FeMo-co biosynthesis. Purified NifQ supported in vitro FeMo-co synthesis in the absence of an additional molybdenum source. The mobilization of molybdenum from NifQ required the simultaneous participation of NifH and NifEN in the in vitro FeMo-co synthesis assay, suggesting that NifQ would be the physiological molybdenum donor to a hypothetical NifEN/NifH complex.es
dc.description.filFil: Hernandez, Jose A.. University of California; Estados Unidos
dc.description.filFil: Curatti, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
dc.description.filFil: Aznar, Constantino P.. University of California; Estados Unidos
dc.description.filFil: Perova, Zinaida. University of California; Estados Unidos
dc.description.filFil: Britt, R. David. University of California; Estados Unidos
dc.description.filFil: Rubio, Luis M.. University of California; Estados Unidos
dc.formatapplication/pdfes
dc.identifier.citationHernandez, Jose A.; Curatti, Leonardo; Aznar, Constantino P.; Perova, Zinaida; Britt, R. David; et al.; Metal trafficking for nitrogen fixation: NifQ donates molybdenum to NifEN/NifH for the biosynthesis of the nitrogenase FeMo-cofactor; National Academy of Sciences; Proceedings of the National Academy of Sciences of The United States of America; 105; 33; 19-8-2008; 11679-11684es
dc.identifier.eissn1091-6490es
dc.identifier.institucionalCONICET
dc.identifier.issn0027-8424es
dc.identifier.repoCONICET Digital
dc.identifier.urihttp://hdl.handle.net/11336/132499
dc.journal.ciudadWashington DCes
dc.journal.number33es
dc.journal.pagination11679-11684es
dc.journal.paisEstados Unidoses
dc.journal.titleProceedings of the National Academy of Sciences of The United States of Americaes
dc.journal.volume105es
dc.language.isoenges
dc.publisherNational Academy of Scienceses
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1073/pnas.0803576105
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/url/https://www.pnas.org/content/105/33/11679
dc.rightsinfo:eu-repo/semantics/openAccess*
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/*
dc.subjectAZOTOBACTER VINELANDIIes
dc.subjectIRON-SULFUR CLUSTERes
dc.subjectNIFes
dc.subject.classificationBiología Celular, Microbiologíaes
dc.subject.classificationCiencias Biológicases
dc.subject.classificationCIENCIAS NATURALES Y EXACTASes
dc.titleMetal trafficking for nitrogen fixation: NifQ donates molybdenum to NifEN/NifH for the biosynthesis of the nitrogenase FeMo-cofactores
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion

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