Plant regeneration from shoot apical meristems of Melia azedarach L. (Meliaceae)

Abstract

A protocol was developed for plani regeneration of MeIia azedarach L. by in vitro culture of meristem (0.5 mm in length). The influence of six clones was investigated. The culture procedure comprised sequential steps: 1 lnduction of shoots by in vitro culture of axillary buds from adult trees (10-15 years old) by culture on Murashige and Skoog (1962) medium (MS) supplemented with 0.5 mg.dm-3 BAP (6-benzylaminopurine), 0.1 mg.dm-3 IBA (indolebutyric acid), and 0,1 mg.dm-3 GA3 (gibberellic acid). The Multiplication of the regenerated shoots was achieved in MS + 0.5 mg.dm-3 BAP + 0.1 mg.dm-3 GA3. 2) In vitro culture of the apical meristems from the regenerated shoots in MS medium (0.7 %) supplemented with various combinations of BAP. Maximum shoot proliferation was obtained on MS medium supplemented with 0.5 mg.dm-3 BAP and 0.1 mg.dm-3 IBA. Regenerated shoots were rooted on MS + 3.5 mg.dm-3 IBA (4 days) followed by subculture on MS laking growth regulators (30 days). Complete plants were transferred to soil.