CD3Ɛ immune restorative ability induced by Maitake Pro4x in immunosupressed BALBc mice

Abstract

Objectives: The aim of this research was to determine if the rich beta glucan compound called Maitake Pro4X can recover the T cell/NK population depleted by Dexamethasone treatment in lymph nodes from cancer-free BALBc female mice. A CD3Ɛ molecular FITC labelled marker was used to measure the effect of Maitake D-Fraction Pro4X (5 mg/kg) on T cell/NK cells populations employing flow cytometry from immunosuppressed female BALBc mice in lymph nodes. There were employed other molecular markers such as CD19, CD105, Ly6G. Results: Maitake Pro4X (5 mg/kg) was able to recover 42.97% of the depleted CD3Ɛ FITC cell population level in Lymph nodes from immunosuppressed female BALBc mice from 4.328±6.229 to 22.646±12.393 (p<0.01) using Flow Cytometry. Maitake was also able to significantly increase the Ly6G PE cell population with p<0.05 in lymph nodes.