Fabrication of a novel electrochemical aptasensor assisted by a novel computerized monitoring system for real-time determination of the prostate specific antigen: a computerized experimental method brought elegancy

Abstract

In this work, a novel and efficient aptasensor assisted by a computerized monitoring system namely SACMES was introduced for real time determination of the prostate specific antigen (PSA). The platform of the aptasensor was a glassy carbon electrode (GCE) modified with graphene quantum dots-chitosan-nafion-ionic liquid/multiwalled carbon nanotubes-graphene-ionic liquid/polypyrrole-MoS2-ionic liquid-AuPt nanoparticles/aptamer (APT/Ppy-MoS2-IL-AuPt NPs/MWCNTs-Gr-IL/GQDs-CS-NAF-IL/GCE). After characterization of the steps of the modification process used to fabricate the aptasensor by electrochemical and microscopic techniques, its square wave voltammetric (SWV) responses at different frequencies as a large-volume of electrochemical data were calibrated with the help of SACMES as an efficient software which helped us to develop an interesting method for real time determination of the PSA. Our records confirmed that the aptasensor response was linearly correlated with PSA concentration in the range of 0.5–350 pg mL−1 with a detection limit of 0.14 pg mL−1. Our results also confirmed that aptasensor was stable, repeatable, reproducible, selective and sensitive for determination of the PSA and its performance in the analysis of real samples was acceptable.