Control of Aspergillus section Flavi growth and aflatoxin accumulation by plant essential oils

Abstract

Aims: The antifungal effect of Pimpinella anisum (anise), Pe¨umus boldus (boldus), Mentha piperita (peppermint), Origanum vulgare (oregano) and Minthosthachys verticillata (peperina) essential oils against Aspergillus section Flavi (two isolates of Aspergillus parasiticus and two isolates of Aspergillus flavus) was evaluated in maize meal extract agar at 0Æ982 and 0Æ955 water activities, at 25C. Methods and Results: The percentage of germination, germ-tube elongation rate, growth rate and aflatoxin B1 (AFB1) accumulation at different essential oils concentrations were evaluated. Anise and boldus essential oils were the most inhibitory at 500 mg kg)1 to all growth parameters of the fungus. These essential oils inhibited the percentage of germination, germ-tube elongation rate and fungal growth. AFB1 accumulation was completely inhibited by anise, boldus and oregano essential oils. Peperina and peppermint essential oils inhibited AFB1 production by 85–90% in all concentrations assayed. Conclusions: Anise and boldus essential oils could be considered as effective fungitoxicans for Aspergillus section flavi. Significance and Impact of the Study: Our results suggest that these phytochemical compounds could be used alone or in conjunction with other substances to control the presence of aflatoxigenic fungi in stored maize.