Emulsan quantitation by Nile red quenching fluorescence assay

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dc.conicet.fuenteindividual
dc.conicet.organizacionConsejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos
dc.conicet.organizacionTufts University. School of Engineering
dc.conicet.organizacionTufts University. School of Engineering
dc.conicet.organizacionTufts University. School of Engineering
dc.conicet.paisOrganizacionArgentina
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.produccionid6290886
dc.contributor.authorCastro, Guillermo Raul
dc.contributor.authorLarson, Bridget K.
dc.contributor.authorPanilaitis, Bruce
dc.contributor.authorKaplan, David L.
dc.date.accessioned2018-04-12T20:50:28Z
dc.date.available2018-04-12T20:50:28Z
dc.date.issued2004-12
dc.date.updated2018-04-10T13:53:36Z
dc.description.abstractA Nile red fluorescent technique to quantify 20–200 μg ml−1 of emulsan was developed. Nile red dissolvedin DMSO showed an adsorption peak at 552 nm, and emission peak at 636 nm, with molar extinction coefficient of 19,600 cm−1 M−1. Nile red fluorescence in DMSO was proportionally quenched by emulsan and the quenching was time-dependent. The assay was used to follow the production of emulsan by cultures of Acinetobacter venetianus RAG-1.es
dc.description.filFil: Castro, Guillermo Raul. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.filFil: Larson, Bridget K.. Tufts University. School of Engineering; Estados Unidos
dc.description.filFil: Panilaitis, Bruce. Tufts University. School of Engineering; Estados Unidos
dc.description.filFil: Kaplan, David L.. Tufts University. School of Engineering; Estados Unidos
dc.formatapplication/pdfes
dc.identifier.citationCastro, Guillermo Raul; Larson, Bridget K.; Panilaitis, Bruce; Kaplan, David L.; Emulsan quantitation by Nile red quenching fluorescence assay; Springer; Applied Microbiology and Biotechnology; 67; 6; 12-2004; 767-770es
dc.identifier.institucionalCONICET
dc.identifier.issn0175-7598es
dc.identifier.repoCONICET Digital
dc.identifier.urihttp://hdl.handle.net/11336/41927
dc.journal.ciudadBerlínes
dc.journal.number6es
dc.journal.pagination767-770es
dc.journal.paisAlemaniaes
dc.journal.titleApplied Microbiology and Biotechnologyes
dc.journal.volume67es
dc.language.isoenges
dc.publisherSpringeres
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00253-004-1849-2
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s00253-004-1849-2
dc.rightsinfo:eu-repo/semantics/restrictedAccess*
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/*
dc.subject.classificationBiología Celular, Microbiologíaes
dc.subject.classificationCiencias Biológicases
dc.subject.classificationCIENCIAS NATURALES Y EXACTASes
dc.titleEmulsan quantitation by Nile red quenching fluorescence assayes
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion

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