Efects of orexin a and b on the expression of its receptors ox1 and ox2 in superovulated rat ovarian cells

Abstract

In previous works we described changes in the orexinergic hypo thalamus-pituitary-ovarian system in adult rats (AJP-EM 292: E820- E828, 2007; AJP-EM, 293: E977-85. 2007). Here we continue to study in vitro in ovaries of superovulated prepubertal rats (SPO) the presence of OX1 and OX2 receptors and the effects of orexin A (OXA) and B (OXB) on their expression. SPO rat ovarian cells were cultured and stimulated for 48 h with OXA (10-9M) and OXB (10-9M) in the presence or absence of their specific antagonists. We evaluated the secretion of progesterone released into the me dium by RIA. The quantification of the receptors was determined by real-time RT-PCR as in AJP-EM 293: E977-85. 2007. [...]