Mycotoxins from beehive pollen mycoflora

Abstract

Aims: The present study was aimed to search for metabolites produced by fungal strains with antifungal activity against phytopathogens. Study Design: The polen of four behives each from two apiaries from two locations in Argentina (Balcarce and Mar del Plata) was studied. Nine be polen samples were randomly colected from each behive. Place and Duration of Study: Study was undertaken during 201 on the Organic Chemistry Department and Biodiversity and Experimental Biology Department, Schol of Exact and Natural Sciences, University of Buenos Aires. Methodology: Al of the 13 isolated strains were tested for antifungal activity. The most bioactive strains, Myrothecium verucaria, Nigrospora sphaerica and a levuliform mycelia, were selected for the isolation and identifcation of compounds responsible for the antifungal activity. Results: Seven mycotoxins, roridin A, verucarin A, α-β-dehydrocurvularin, phomalactones A, B, C and D, were identifed spectroscopicaly and were the responsible the antifungal activity. Conclusion: Mycotoxins were isolated from behive polen mycoflora. This is the first report of the production of these mycotoxins, which are not curently targets for the analysis of polen or honey products.