Validation of ERETIC‐Based 1 H‐NMR for the Simultaneous Quantification of Cannabinoids in Complex Cannabis sativa Extracts

Abstract

Chromatographic methods for cannabinoid analysis currently face limitations, including lengthy procedures, artifact formation, and reliance on reference standards. This study establishes a robust quantitative 1H-NMR methodology for simultaneous analysis of acidic and neutral cannabinoids (THCA, CBDA, Δ9-THC, CBD, and CBN) in Cannabis sativa extracts. Two validated strategies, internal standard (butylated hydroxytoluene [BHT]) and ERETIC calibration, were compared, with diagnostic signals identified in interference-free spectral regions. The method demonstrated high precision (RSD<5%), accuracy (recoveries 97%–102%), and low detection limits (MLOD 0.07mg/mL). ERETIC outperformed traditional calibration by eliminating physical standards while maintaining equivalency. Successful chemotypic classification (THC:CBD ratios) confirmed practical applicability. The ERETIC-based NMR approach provides a rapid and reproducible alternative for comprehensive cannabinoid quantification without derivatization.