Studies of ciprofloxacin encapsulation on alginate/pectin matrixes and its relationship with biodisponibility

Abstract

Screening of ciprofloxacin (Cip) with selected biopolymers brings about 90% antibiotic interactions with a coacervate composed of alginate/high metoxylated pectin in 2:1 ratio. Fourier transform infrared spectroscopy analysis provides information about the nature of this interaction, revealing ionic and hydrophobic patterns among the molecules. Alginate/ high methoxylated pectin gel microspheres developed by ionic gelation encapsulates 46.8± 5.0% Cip. The gel matrix can release Cip in a sustained manner, releasing 42.7±0.2% in 2 h under simulated stomach pH conditions, and 83.3±1.1% Cip release in 80 mM phosphate at pH07.40 (intestinal). The increase of sodium chloride from 50 to 200 mM implies a Cip release from 69.0±1.5% to 95.1±3.6% respectively in 2 h. Scanning electron microscopy revealed the cohesive effect of HM pectin over alginate molecules on the microsphere surface. Those results guarantee all Cip contained in the alginate/HM pectin microspheres could be released in an established kinetic profile along the gastrointestinal tract, avoiding the Cip undesirable side effects during absorption.