Tiny hybrid modified organosilane-titanium dioxide nanocomposites with dual photonic behavior: insights for enhanced in-flow signaling

Abstract

In this Research work it was studied spectroscopical properties of tiny TiO2 Nanoparticles with potential applications by the stimulation of multi-modal energy modes. Thus, Nanoparticles of 2-4 nm sizes were modified with short modified organosilane with a Fluorescent Laser dye reporter. By this manner, it was covered with silanized spacer lengths of few nm with an additional protective shell. The non-modified TiO2 Nanoparticles showed high interactions between them due to their reduced sizes that afforded to homogeneous Nano-patterned arrays observed by TEM. While, silanized Nanoparticles afforded to higher dispersible colloidal dispersion with strong interactions as well. As it is known silanol groups could form strong polar and hydrogen bridges between them, but the aggregates could being easily re-dispersed just by sonication. By this manner, it was lost a high ordered Nano-pattern generated in absence of silica shells. By Dynamic Light Scattering (DLS). it was recorded within colloidal dispersions sizes of 2-3 nm in presence of aqueous solvent; and smaller Nanoaggregates of 30 nm corresponding to decamer forms. In this context, it was highlighted the possibility to manage interactions and Nano-patterns controlling Nano-surfaces and media.Non-labelled TiO2 Nanoparticles showed strong absorptions centred at 298.0 nm and 560.0 nm. Moreover, modified Nanoparticles showed a constant and proportional increase of the 298.0 nm absorption band with the augmentation of the concentration. Moreover the UV band it was accompanied with a redshifted maximal absorption wavelength by the fluorophore incorporated from 553.0 nm to 557.0 nm. This fact was explained between an interaction between the absorption of the Laser dye centred at 537 nm and the longer band by the Nanoplatforms. Then in order to evaluate their interactions in the excited state, it was evaluated Fluorescence Lifetime Decays (τ) of the free Fluorophore and deposed on Nanoplatforms. In addition it was evaluated the detection of the modified TiO2 Nanoplatforms within In Flow Cytometry (IFC). In this manner, it was observed τ shortening accompanied with augmented counting of Fluorescence events detected. It should be noted that in these conditions it was not observed degradation of the Fluorescent reporter. However in absence of the modified organolsilanes; it was observed potential insights of fast photo-degradation under UV light irradiation.Therefore, it was designed and synthesized a stable and improved tiny Nano-emitter with potential use for Nanophotonics, Biophotonics, and Nanomedicine applications. Thus, in this perspective it was discussed the development of a Multimodal Nanoplatforms for varied functionalities and applications.