Development of an inverse-PCR approach for characterization of the major BCR-ABL1 breakpoint sequences on genomic DNA: Proof of concept

Abstract

Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by the presence of the Philadelphia chromosome, which contains the BCR-ABL1 fusion gene. The international conventional standardized method for routine monitoring of CML patients under tyrosine kinase inhibitor therapy is based on real-time quantitative reverse transcription-PCR (qRT-PCR) of BCR-ABL1...