Lipid bilayer patterns fabrication by one-photon lithograpy

Abstract

In the last decade, there has been an increasing interest in patterning surfaces with supported lipid bilayers. The ability of this system to control molecular composition and mobility of this system at micro- and nanoscales have inspired several new routes of biological and biotechnological investigation. Successes in this field include the development of photolithographic and soft lithography techniques which allow the printing of a variety of biomolecules on different substrates. Here, we describe a simple, flexible, and inexpensive protocol for the generation of lipid bilayer micropatterns based on one-photon lithography and microcontact printing (μCP).