Could probiotics be used as a novel therapeutic approach to alleviate the reproductive and neurobehavioral side effects of sertraline? A study in male mice

Abstract

There are still few studies that have investigated the impact of sertraline (SE) on fertility, as well as adjuvanttreatments that alleviate its side effects. Thus, the present study aimed to investigate the impact of SE onreproductive and neurobehavior parameters and verify whether the probiotic Lactobacillus rhamnosus alleviatesthe side effects of SE. After carrying out a dose-response study with SE, experiment II was conducted. Thus, malemice were distributed into four experimental groups (n=8–9/group): control (CO)-received filtered water(vehicle); sertraline group (S)–received 20 mg/kg of SE, diluted in the vehicle; probiotic (P)-received the probioticLactobacillus rhamnosus (1×109 CFU) diluted in the vehicle; and SP group that received both probiotic andSE. The treatment occurred/lasted for 30 days. At the end of treatment, behavioral aspects were analyzed. Aftereuthanasia, the organs were weighed, the histology of the testis and epididymis were analyzed, and the spermquality and also natural fertility were verified. Complementarily, in vitro assays were carried out to verifywhether sertraline could affect sperm capacitation and embryonic development. The results showed that the SPgroup, compared to S, did not reduce body weight and seminal gland weight and presented a lower number ofresorptions. Notably, the rate of resorption in both the SP and S groups was similar to that of the control group. Itwas also observed that the S group was less exploratory and more anxious than the SP group. Thus, the presentstudy demonstrated that SE has an impact on the reproductive system and neurobehavior. Therefore, for the firsttime, we demonstrate that the probiotic can alleviate the side effects of SE.