Digestive and metabolic profile at the biochemical level of juvenile flounder Paralichthys orbignyanus (Valenciennes, 1839) (Pleuronectiformes: Paralichthyidae)
; Radonic, Mariela ; López, Andrea ; Lopez Mañanes, Alejandra Antonia 
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12/2016
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PanamJAS
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Pan-American Journal of Aquatic Sciences
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1809-9009
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El lenguado Paralichthys orbignyanus el cual se distribuye desde Rio de Janeiro (Brasil) hasta el Golfo de San Matías, Argentina, desempeña un importante rol ecológico y es de gran potencial para acuicultura. Sin embargo, faltan estudios sobre fisiología digestiva (ej. existencia de enzimas digestivas clave), y sobre sitios de reserva de energía. Determinamos la existencia y características de amilasa, maltasa, sacarasa, lipasa y N-aminopeptidasa (APN) en tracto digestivo de juveniles como índice de capacidad de digerir sustratos dietarios glucogénicos y lipídicos y para digestión final de proteínas y el contenido de glucógeno, triglicéridos y proteínas en diferentes tejidos para identificar sitios de almacenamiento. La existencia, características y la distribución en tracto digestivo de las actividades de amilasa, maltasa, sacarasa, lipasa y APN y el contenido de glucógeno, triglicéridos y proteínas sugiere que juveniles de P. orbignyanus exhiben una adecuada batería digestiva para la hidrólisis completa de varios sustratos dietarios y capacidad para almacenamiento y/o utilización de reservas de energía en hígado y músculo. Los resultados sugieren además que juveniles de P. orbignyanus soportarían una dieta con alto contenido de carbohidratos, lo que podría representar una ventaja para su posible aplicación en la producción comercial debido al menor costo de la dietas.
The flounder Paralichthys orbignyanus that spans from Rio de Janeiro (Brazil) toSan Matías Gulf, Argentina has an important ecological role and a great potential use foraquaculture. However, studies on its digestive physiology (i.e. occurrence of digestiveenzymes) as well on energy reserves content in metabolic tissues are lacking. We determined theoccurrence and characteristics of amylase, maltase, sucrase, lipase and aminopeptidase-N indigestive tract of juveniles as an index of the ability to digest dietary glycogenic substrates from different sources, lipids and for final protein digestion. Glycogen, triglycerides and proteincontent in different tissues were also analyzed to identify storage sites. Presence and distribution in the digestive tract of the enzymes amylase, maltase, sucrase, lipase and N-aminopeptidase, aswell as glycogen, triglycerides and protein suggest that juveniles of P. orbignyanus exhibit anadequate digestive battery to potentially perform complete hydrolysis of various dietarysubstrates and capacity for storage and/or utilization of energy reserves in liver and muscle. Moreover, our results show that juveniles of P. orbignyanus could accept a diet with highcarbohydrates content, which could represent and advantage for its potential application in fishfarming due to the possibility to use cheaper diets.Keywords: flatfishes, digestive enzymes, energy reserves.
The flounder Paralichthys orbignyanus that spans from Rio de Janeiro (Brazil) toSan Matías Gulf, Argentina has an important ecological role and a great potential use foraquaculture. However, studies on its digestive physiology (i.e. occurrence of digestiveenzymes) as well on energy reserves content in metabolic tissues are lacking. We determined theoccurrence and characteristics of amylase, maltase, sucrase, lipase and aminopeptidase-N indigestive tract of juveniles as an index of the ability to digest dietary glycogenic substrates from different sources, lipids and for final protein digestion. Glycogen, triglycerides and proteincontent in different tissues were also analyzed to identify storage sites. Presence and distribution in the digestive tract of the enzymes amylase, maltase, sucrase, lipase and N-aminopeptidase, aswell as glycogen, triglycerides and protein suggest that juveniles of P. orbignyanus exhibit anadequate digestive battery to potentially perform complete hydrolysis of various dietarysubstrates and capacity for storage and/or utilization of energy reserves in liver and muscle. Moreover, our results show that juveniles of P. orbignyanus could accept a diet with highcarbohydrates content, which could represent and advantage for its potential application in fishfarming due to the possibility to use cheaper diets.Keywords: flatfishes, digestive enzymes, energy reserves.
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