Salinization and low-dose levels of pesticides alter brain shape of larval amphibians

Abstract

Wetland communities are increasingly threatened by multiple stressors simultaneously, such as pesticides and salinization. We examined the effects of ecologically-relevant exposures to broad-spectrum insecticides and salinization on amphibian neurodevelopment, which is strongly linked to how organisms respond behaviorally to environmental change. Prior research showed that exposure to trace concentrations of an organophosphate pesticide (chlorpyrifos) altered the brain shape and behavior of larval and metamorphic amphibians. It is unknown whether brain shape is altered by additional pesticides and road salt. Using outdoor mesocosms, we tested whether salt (NaCl) and representatives from three pesticide families (organophosphates, pyrethroids, andneonicotinoids) altered tadpole (Lithobates pipiens) brain shape. Of the two organophosphates, chlorpyrifos induced relatively longer telencephalon lengths relative to body mass, consistent with previous studies, but malathion had no effect on brain shape. Of the two pyrethroids, permethrin, but not cypermethrin, increased telencephalon length. For the neonicotinoids, there were marginally significant effects of imidacloprid and thiamethoxam on telencephalon length. Thus, the impacts of pesticides on brain shape was not dictated by pesticide family. Exposure to relatively high concentrations of salt resulted in brains that were less wide but had longer optic tecta. Although we failed to find strong interactive effect of salt with pesticides, there was some weak, nonsignificant, evidence that exposure to salt masked responses to pesticides. Together, our results indicate that environmentally realistic levels of pesticides and salinization can alter larval brain shape. Our study highlights the importance of studying the impacts of naturally-occurring levels of pesticides and salinization on vertebrate neural development.