Remoción de cromo hexavalente de aguas residuales con microorganismos adaptados a medios ricos en cromo
; Landazuri, Patrici ; Loango, Nelsy Otros responsables
Fecha
10/2017
Editorial
Asociación Colombiana de Ciencias Biológicas
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Revista de la Asociación Colombiana de Ciencias Biológicas
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2500-7459
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Resumen
Los problemas de contaminación de acuíferos a nivel mundial son motivo de preocupación y requieren métodos efi caces de remediación que permitan minimizar el impacto ambiental generado por vertimientos industriales y domésticos. En el presente estudio se determinó la capacidad de remoción de Cr6+ en bacterias y levaduras adaptadas a concentraciones altas de este metal. Métodos: Los ensayos de remoción se llevaron a cabo en medio de cultivo BHI y en agua residual (AR) a los cuales se les adicionó dicromato de potasio (K2Cr2O7). Resultados: Las bacterias y levaduras aisladas y adaptadas a Cr6+ demostraron ser reductoras de este metal. Los aislados bacterianos Raoultella sp, Serratia sp y Klebsiella sp presentaron mayor reducción de Cr6+ en AR alcanzando el 100% de reducción de cromo en 30 horas, estos tres aislados se destacaron por su capacidad de reducir Cr6+ tanto en medio de cultivo como en agua residual en todas las concentraciones de K2Cr2O7 evaluadas. Los aislados levaduriformes Candida Tropicalis, Candida Famata y Cryptococus neoformans no presentaron diferencias significativas con el control en el ensayo de reducción del metal en AR con diferentes concentraciones de Cr6+. Conclusiones: La capacidad de reducción del Cr6+, así como el crecimiento bacteriano en condiciones altas de este metal, permiten proponer a Klebsiella sp, Raoultella sp, y Serratia sp como microorganismos promisorio para la bioremediación de sitios contaminados con Cr6+ a escala real.
Global aquifer contamination problems are a cause for concern and require effective remediation methods to minimize the environmental impact generated by industrial and domestic dumping. In the present study, the ability to remove Cr6+ from bacteria and yeasts adapted to high concentrations of this metal was determined.Methods: Removal tests were carried out in BHI culture medium and in residual water to which potassium dichromate (K2Cr2O7) was added. Results: Bacteria and yeasts isolated and adapted to Cr6+ were shown to be reductive of this metal. The bacterial isolates Raoultella sp, Serratia sp and Klebsiella sp presented higher reduction of Cr6+ in residual water (AR) reaching 100% reduction of chromium in 30 hours, these three isolates were highlighted by their ability to reduce Cr6+ in both culture medium As in residual water at all concentrations of K2Cr2O7 evaluated. The yeast isolates Candida Tropicalis, Candida Famata and Cryptococus neoformans did not present signifi cant differences with the control in the reduction test of the metal in RA with different concentrations of Cr6+. Conclusions: The ability of Cr6+ reduction, as well as the bacterial growth in high conditions of this metal, allow to propose Klebsiella sp, Raoultella sp, and Serratia sp as promising microorganisms for the bioremediation of sites contaminated with Cr6+ in real scale.
Global aquifer contamination problems are a cause for concern and require effective remediation methods to minimize the environmental impact generated by industrial and domestic dumping. In the present study, the ability to remove Cr6+ from bacteria and yeasts adapted to high concentrations of this metal was determined.Methods: Removal tests were carried out in BHI culture medium and in residual water to which potassium dichromate (K2Cr2O7) was added. Results: Bacteria and yeasts isolated and adapted to Cr6+ were shown to be reductive of this metal. The bacterial isolates Raoultella sp, Serratia sp and Klebsiella sp presented higher reduction of Cr6+ in residual water (AR) reaching 100% reduction of chromium in 30 hours, these three isolates were highlighted by their ability to reduce Cr6+ in both culture medium As in residual water at all concentrations of K2Cr2O7 evaluated. The yeast isolates Candida Tropicalis, Candida Famata and Cryptococus neoformans did not present signifi cant differences with the control in the reduction test of the metal in RA with different concentrations of Cr6+. Conclusions: The ability of Cr6+ reduction, as well as the bacterial growth in high conditions of this metal, allow to propose Klebsiella sp, Raoultella sp, and Serratia sp as promising microorganisms for the bioremediation of sites contaminated with Cr6+ in real scale.
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