High resolution films for bone regeneration evaluation

Abstract

Diagnostic imaging techniques (DIxT) seem to be a useful tool for evaluating bone formation in both human and animal models. There is little evidence on the use of Soft X-Rays (sXR) with high-resolution films for studying the healing process in critical bone size defects (CSD). The aim of this study was to evaluate the ability of soft X-Ray – High Resolution Films (sXR) to distinguish bone regeneration in CSDs. A CSD was created in each of 16 Wistar rat calvariae. The animals were euthanized at 1, 3 and 6 weeks after surgery. The samples were submitted to cXR (conventional X-rays), sXR techniques and histological procedures (HP). Bone formation was observed at CSD edges at all periods of time. At 6 week, there was also new bone in the central area. The CSD was not fully regenerated after any period of time. Histometric results were 0.16%; 0.75% and 0.89% new bone formed at weeks 1, 3 and 6 respectively; radiometric results at cXR were 0% in all samples. Evaluation of sXR shows 0.4%; 0.50% and 3.64% bone at weeks 1, 3 and 6. Mean and Standard Deviation were calculated. The data were submitted to statistical analysis using the Pearson product-moment correlation coefficient test. The r value was 0.581. Under these experimental conditions, sXR was found to be a suitable method for detecting new bone formation, based on the positive correlation between sXR and HP during the bone healing process of CSDs in rat calvaria. Furthermore, the sXR technique allowed us to obtain samples with appropriate spatial orientation.