The Botrytis cinerea aspartic proteinase family

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dc.conicet.organizacionConsejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas
dc.conicet.organizacionUniversidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales
dc.conicet.organizacionWageningen University. Laboratory of Phytopathology
dc.conicet.organizacionUniversidad de la Laguna
dc.conicet.organizacionWageningen University. Laboratory of Phytopathology
dc.conicet.organizacionThe Australian Wine Research Institute
dc.conicet.organizacionThe University Of Melbourne
dc.conicet.organizacionUniversidad de la Laguna
dc.conicet.organizacionCardiff University
dc.conicet.organizacionUniversidad de la Laguna
dc.conicet.organizacionWageningen University. Laboratory of Phytopathology
dc.conicet.paisOrganizacionArgentina
dc.conicet.paisOrganizacionArgentina
dc.conicet.paisOrganizacionPaíses Bajos
dc.conicet.paisOrganizacionEspaña
dc.conicet.paisOrganizacionPaíses Bajos
dc.conicet.paisOrganizacionAustralia
dc.conicet.paisOrganizacionAustralia
dc.conicet.paisOrganizacionEspaña
dc.conicet.paisOrganizacionReino Unido
dc.conicet.paisOrganizacionEspaña
dc.conicet.paisOrganizacionPaíses Bajos
dc.conicet.produccionid5679373
dc.contributor.authorTen Have, Arjen
dc.contributor.authorEspino, José J.
dc.contributor.authorDekkers, Ester
dc.contributor.authorVan Sluyter, Steven C.
dc.contributor.authorBrito, Nélida
dc.contributor.authorKay, John
dc.contributor.authorGonzález, Celedonio
dc.contributor.authorvan Kan, Jan A. L.
dc.date.accessioned2017-02-21T17:25:12Z
dc.date.available2017-02-21T17:25:12Z
dc.date.issued2010-12
dc.date.updated2017-02-21T14:38:12Z
dc.description.abstractThe ascomycete plant pathogen Botrytis cinerea secretes aspartic proteinase (AP) activity. Functional analysis was carried out on five aspartic proteinase genes (Bcap1-5) reported previously. Single and double mutants lacking these five genes showed neither a reduced secreted proteolytic activity, nor a reduction in virulence and they showed no alteration in sensitivity to antifungal proteins purified from grape juice. Scrutiny of the B. cinerea genome revealed the presence of nine additional Bcap genes, denoted Bcap6-14. The product of the Bcap8 gene was found to constitute up to 23% of the total protein secreted by B. cinerea. Bcap8-deficient mutants secreted approximately 70% less AP activity but were just as virulent as the wild-type strain. Phylogenetic analysis showed that Bcap8 has orthologs in many basidiomycetes but only few ascomycetes including the biocontrol fungus Trichoderma harzanium. Potential functions of the 14 APs in B. cinerea are discussed based on their sequence characteristics, phylogeny and predicted localization.es
dc.description.filFil: Ten Have, Arjen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Wageningen University. Laboratory of Phytopathology; Países Bajos
dc.description.filFil: Espino, José J.. Universidad de la Laguna; España
dc.description.filFil: Dekkers, Ester. Wageningen University. Laboratory of Phytopathology; Países Bajos
dc.description.filFil: Van Sluyter, Steven C.. The Australian Wine Research Institute; Australia. The University Of Melbourne; Australia
dc.description.filFil: Brito, Nélida. Universidad de la Laguna; España
dc.description.filFil: Kay, John. Cardiff University; Reino Unido
dc.description.filFil: González, Celedonio. Universidad de la Laguna; España
dc.description.filFil: van Kan, Jan A. L.. Wageningen University. Laboratory of Phytopathology; Países Bajos
dc.formatapplication/pdfes
dc.identifier.citationTen Have, Arjen; Espino, José J.; Dekkers, Ester; Van Sluyter, Steven C.; Brito, Nélida; et al.; The Botrytis cinerea aspartic proteinase family; Elsevier Inc; Fungal Genetics And Biology; 47; 1; 12-2010; 53-65es
dc.identifier.issn1087-1845
dc.identifier.urihttp://hdl.handle.net/11336/13256
dc.journal.ciudadAmsterdames
dc.journal.number1es
dc.journal.pagination53-65es
dc.journal.paisPaíses Bajoses
dc.journal.titleFungal Genetics And Biologyes
dc.journal.volume47es
dc.language.isoenges
dc.publisherElsevier Inces
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1087184509001765
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.fgb.2009.10.008
dc.rightsinfo:eu-repo/semantics/openAccess*
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/es
dc.subjectGray Mouldes
dc.subjectBioinformaticses
dc.subjectProteinasees
dc.subjectPlant Pathogenes
dc.subjectPhylogenyes
dc.subjectEvolutiones
dc.subject.classificationBioquímica y Biología Moleculares
dc.subject.classificationCiencias Biológicases
dc.subject.classificationCIENCIAS NATURALES Y EXACTASes
dc.titleThe Botrytis cinerea aspartic proteinase familyes
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion

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