Molecular phylogenetic relationship between Philometroides tahieli (Nematoda, Philometridae) and other philometrids from South America

Abstract

In South America, the family Philometridae is represented by several genera and species. In particular, Philometra and Philometroides are the most speciose genera. In Argentina, only Philometroides tahieli has been reported so far. The main objective of this study was to analyze the phylogenetic relationship between Ps. tahieli and other South American philometrids using molecular data. The molecular analysis was performed using a female specimen of Ps. tahieli found on the opercular muscle of a juvenile Micropogonias furnieri from brackish waters in Argentina. Phylogenetic relationships were studied based on partial sequences of the 18S rDNA and 28S rDNA genes. In the 18S rDNA tree, Ps. tahieli was mainly grouped with other Philometroides from freshwater hosts from China, sharing the site of infection (head tissues or muscles). In the 28S rDNA tree, obtained with fewer sequences, Ps. tahieli is related species from subcutaneous tissues of head. This study contributes with information on philometrids and confirms the presence of different lineages among South American species, with Ps. tahieli representing a new one. Further studies on South American species using more molecular markers and new morphological characters will improve our knowledge of philometrid biodiversity and phylogeny.