Diversity of fungal communities inhabiting symptomatic leaves of Cape gooseberry (Physalis peruviana) in the Ecuadorian Andes
; Yánez, Jennifer Otros responsables
Fecha
11/2019
Editorial
Pontificia Universidad Católica del Ecuador
Revista
Revista Ecuatoriana de Medicina y Ciencias Biológicas
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ISSN
2477-9113
eISSN
2477-9148
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Resumen
Utilizamos métodos cultivo-dependientes para evaluar la ocurrencia y diversidad de comunidades fúngicas que habitan en tejido foliar sintomático de uvilla (Physalis peruviana) en cuatro localidades del norte de los Andes ecuatorianos. Categorizamos los aislados basándonos en las características morfológicas de los cultivos obtenidos in vitro, los cuales identificamos al secuenciar la región de ADNr ITS. En total, recuperamos un total de 75 hongos ascomycetos que comprendían 13 géneros y 20 taxa. Encontramos que la mayoría de hongos recuperados de tejidos necróticos o cloróticos pertenecían al género Alternaria. Otros taxa que encontramos con frecuencia fueron Epicoccum, Diaporthe y Xylaria. A nuestro conocimiento, este es el primer estudio en reportar la micobiota asociada a plantas de P. peruviana que presenten hojas con síntomas de enfermedad, incluyendo 2 géneros de hongos nuevos para el Ecuador. Esperamos que nuestros resultados provean información útil para futuros estudios sobre control biológico aplicado a cultivos comerciales en los trópicos.
We used microbial culture specific methods to assess the occurrence and diversity of fungal communitiesinhabiting symptomatic foliar tissue of Cape gooseberry (Physalis peruviana) at four different field plotsin the northern EcuadorianAndes. We categorized the isolated fungi based on their morphological characteristics and subsequently identified them through rDNA sequencing of the ITS region. We recovered 75 ascomycetous isolates which represented 13 genera, revealing a total of 20 taxa. We found most isolates recovered from necrotic and straw-colored tissues to belong to the genus Alternaria. Other fungal taxa frequently found were Epicoccum, Diaporthe, and Xylaria. To our knowledge, this is the first study to report the mycobiota associated to P. peruviana plants showing disease symptoms, including 2 new genera of fungi for the first time in Ecuador. Our results are expected to provide useful information for future assessments of biological control on tropical commercial.
We used microbial culture specific methods to assess the occurrence and diversity of fungal communitiesinhabiting symptomatic foliar tissue of Cape gooseberry (Physalis peruviana) at four different field plotsin the northern EcuadorianAndes. We categorized the isolated fungi based on their morphological characteristics and subsequently identified them through rDNA sequencing of the ITS region. We recovered 75 ascomycetous isolates which represented 13 genera, revealing a total of 20 taxa. We found most isolates recovered from necrotic and straw-colored tissues to belong to the genus Alternaria. Other fungal taxa frequently found were Epicoccum, Diaporthe, and Xylaria. To our knowledge, this is the first study to report the mycobiota associated to P. peruviana plants showing disease symptoms, including 2 new genera of fungi for the first time in Ecuador. Our results are expected to provide useful information for future assessments of biological control on tropical commercial.
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