Manganese Cytotoxicity Assay on Hippocampal Neuronal Cell Culture

Abstract

Compared to an in vivo experiment, neuronal cell cultures are immediately accessible<br />to observation and manipulation. In this protocol, we describe a technique to evaluate the<br />cytotoxicity of a metal, manganese (Mn2+), on hippocampal neuronal cell cultures. Interestingly, this protocol is easily adaptable to any type of primary culture (e.g., cortical neurons) and any type of toxic compound (e.g., chemical product).