Improved method for separation of high molecular weight DNA molecules from Zymomonas mobilis
; Siñeriz, Faustino ; Abate, Carlos Mauricio Otros responsables
Fecha
12/2000
Editorial
Springer
Revista
Biotechnology Letters
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0141-5492
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Resumen
An improved method for separation of large DNA molecules from Zymomonas mobilis was developed using a contour-clampe homogeneous electric field technique. The preparation of DNA samples was optimized, as well as the migration conditions, to resolve the chromosomal DNA fractions obtained after digestion with SfiI. A 22s (45H) pulse setting gave maximal separation of 240 to 109 kb fragments range, while 9 s (40h), 3s (35h) and 1 s (30h) settings produced the best separation of 120 to 64 kb, 64 to 34 kb fragments range and less than 35 kb fragments, respectively.
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