Effect of Light Conditions on Growth and Antioxidant Parameters of Two Hydroponically Grown Lettuce Cultivars (Green and Purple) in a Vertical Farm System

Abstract

The use of extended light spectra, including UV-A, green, and far-red, hasbeen scarcely explored in vertical farming. This study evaluated the effects of full spectra under two intensities (90 and 180 µmol m−2s−1) on the growth and antioxidant properties of green and purple leaf lettuce. Three light spectra were tested: Blue-White (BW), Red-White (RW), and Red-Blue (RB). Fresh weight (FW), dry weight percentage (DWP), chlorophyll concentration (NDVI), and antioxidant parameters (total phenoliccontent (TPC), antioxidant capacity by DPPH and FRAP and total flavonoid content (TFC)) were assessed. Spectrum-intensity interactions significantly influenced FW, with RW-180 µmol m−2 s −1 yielding the highest FW (78.2 g plant−1 in green and 48.5 g plant−1in purple lettuce). BW-90 µmol m−2 s −1 maximized DWP in green lettuce, while PARintensity of 180 µmol m−2s−1 favored DWP in purple lettuce. Chlorophyll concentrationincreased under PAR intensity of 180 µmol m−2 s−1 , and leaf color varied with spectrum,with RW producing lighter leaves. Antioxidant parameters declined over time, but a PARintensity of 180 µmol m−2 s−1 , particularly under RW, boosted TPC and TFC contentsin both lettuce cultivars during early stages (days 0 and 15). Conversely, a lower PARintensity of 90 µmol m−2s−1 , mainly under RW, enhanced antioxidant capacity by FRAPat 15 days and by the end of the cycle for both cultivars. Overall, RW-180 µmol m−2s−1interactions promoted the best characteristics in lettuce. Nonetheless, the findings emphasizethe significance of fine-tuning both light spectrum and intensity to enhance lettuce growthand quality in vertical farming systems considering the cultivar, time and variable tobe evaluated.