Bioproduction of Floxuridine Using Nanostabilized Biocatalysts

Abstract

Bionanocomposites employing natural polysaccharides such as alginate and nanoclays, e.g., bentonite, are a promising alternative to developing stabilized biocatalysts used in the pharmaceutical industry due to their physicochemical properties, biocompatibility, and nontoxicity. Mechanical parameters such as swelling ratio, compressive strength, and fracture frequency were optimized, favoring scale-up. Moreover, storage stability and reusability of the biocatalysts were improved by more than 90 % compared with control conditions. In addition, immobilized lactic acid bacteria were used in the bioprocess scale-up to obtain floxuridine, showing a high productivity per gram of biocatalyst.