Effects of the use of tannins on the ruminal fermentation of cattle: A meta-analysis and meta-regression

Abstract

This systematic review-meta-analysis (MA) estimates the impact of including tannins in beef and dairy cattle diets on short-chain fatty acids (SCFA), ammonia nitrogen (N-NH3), dry matter di- gestibility (DMD), and methane (CH4) production. The effects are described according to the sources (natural or extract), type of tannin (condensed, CT, or hydrolyzed HT or blend), total tannin concentration in the source, and doses. The bibliographic search was performed in five electronic databases: CAB-Abstracts, PubMed, Scopus, Science Direct, and Web of Science. Pre- defined protocols were used at all stages of the systematic review process. An MA for random effects was performed for each indicator separately with the mean difference between the control (without tannin) and treated (with tannin) groups. Twenty-seven articles were included for this study, of which 20 were for SCFA, 20 for N-NH3, 14 for DMD, and 10 for CH4. The inclusion of HT reduced the total SCFA concentration (p = 0.005). Within a dose range of 10–40 g/kg of a CT extract, the total SCFA concentration was reduced by 0.804 mM in the rumen fluid (p < 0.016). The effects of tannins on reducing the N-NH3 concentration are evident, either extracted or naturally in the plant, and regardless of the type of tannin (p < 0.05). DMD was reduced with the use of a tannin blend (p = 0.041) and the inclusion of tannins reduced CH4 production (p = 0.005), with moderate heterogeneity (I2 = 44.5 %). Through the 27 studies included, we concluded that tannins affect the concentration of N-NH3, SCFA, DMD, and CH4. Tannins asso- ciated with balanced protein-energy diets can reduce ruminal protein degradability and CH4 production, which can benefit animal metabolism and the environment.