Total free lipids from MDR strain of Mycobacterium tuberculosis “M” reduce T cell activation and CTL activity in healthy individuals

Abstract

Increasing evidence highlights the role of cell wall components in the effectiveness of different Mycobacteriumtuberculosis (Mtb) strains in modulating host immune response. We previously demonstrated that the outbreakmultidrug-resistant strain M displays a distinctive lipid profile in its cell envelope compared to the closely relatedsporadic strain 410. Both strains markedly differ in their ability to induce fully functional CD8+ T cells because oflow CD69 signaling and impaired CD4+ T cell help. In this study, we evaluated the impact of extractable lipids(LP) from M (LP-M) and 410 (LP-410) on the activation and functionality of T cells from healthy individuals.PBMCs were cultured alone or with Mtb in the presence or absence of LP-M, LP-410, or LP from CD1551 mutantsin polymorphic genes between M and 410. Then, surface CD69 and intracytoplasmic IL-2 (after 3 days of culture), as well as surface CD107 expression (after 6 days of culture) were determined in T cells by flow cytometry.In contrast to LP-410, LP-M induced low expression of CD69 and IL-2 in CD4+/CD8+ cells and of CD107 in CD8+cells. Besides, LP from Mtb strains mutated in Rv1861c and Rv3787c genes inhibited H37Rv-induced T cellresponse without causing cell death. Thus, our results suggest that LP-M likely through mutations in Rv1861 andRv3787c, inhibits the activation and functionality of T cells from PPD+ healthy human donors and mightpartially contribute to the development of immune evasion mechanisms in the M strain.