Artículo

A simply method for the addition of rotenone in Arabidopsis thaliana leaves

 Maliandi, María Victoria Icon; Rius, Sebastian Pablo Icon; Busi, María Victoria Icon; Gomez Casati, Diego Fabian Icon

Otros responsables

Fecha

07/2015

Editorial

Taylor & Francis

Revista

Plant Signaling and Behavior

Título del libro

Nombre del evento

Fecha del evento

Institución organizadora

ISSN

1559-2316

eISSN

ISBN

Idioma

Inglés

Tipo del evento

Clasificación temática

Bioquímica y Biología Molecular

Palabras clave

Rotenone , Arabidopsis Thaliana , Mitochondria , Mitochondrial Respiration , Complex I

Resumen

A simple and reproducible method for the treatment of Arabidopsis thalianaleaves with rotenone is presented. Rosette leaves were incubated with rotenone andTriton X-100 for at least 15 h. Treated leaves showed increased expression of COX19 and BCS1a, two genes known to be induced in Arabidopsis cell cultures afterrotenone treatment. Moreover, rotenone/Triton X-100 incubated leaves presented aninhibition of oxygen uptake. The simplicity of the procedure shows this methodology useful for studying the effect of the addition of rotenone to a photosynthetic tissue in situ.

URI

http://hdl.handle.net/11336/7826

Colecciones

Articulos(CEFOBI)

Aprobación

Revisión

Complementado por

Referenciado por

Licencia Creative Commons

by-nc-sa
Excepto donde se indique lo contrario, la licencia de este ítem se describe como by-nc-sa
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Miniatura

Archivos

Principal CONICET_Digital_Nro.6434_A.pdf (194.29 KB)
Identificadores
URI: http://hdl.handle.net/11336/7826
DOI: http://www.tandfonline.com/doi/full/10.1080/15592324.2015.1073871
URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4883935/
Citación
Maliandi, María Victoria; Rius, Sebastian Pablo; Busi, María Victoria; Gomez Casati, Diego Fabian; A simply method for the addition of rotenone in Arabidopsis thaliana leaves; Taylor & Francis; Plant Signaling and Behavior; 10; 10; 7-2015; 1-3
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