Chaotic fluctuations in mitochondrial function under oxidative stress. Superoxide dismutatase (SOD2) concentrations of 0.013, 0.016,0.0164, 0.0216733 mM
; Aon, Miguel Antonio ; Cortassa, Sonia del Carmen ; Lloyd, David ; Sollott, Steven Otros responsables
Fecha
Publicador:
Consejo Nacional de Investigaciones Científicas y Técnicas
Fecha de depósito:
18/04/2022
Fecha de creación:
01/01/2017-31/12/2017
Clasificación temática
Palabras clave
Resumen
These chaotic time series of mitochondrial dynamics under conditions of oxidative stress are analyzed in detail in Kembro et al. 2018. Mitochondrial chaotic dynamics: Redox-energetic behavior at the edge of stability. Mitochondrial model used was described in the project overview and in detail (Kembro et al. 2013. Biophys J 104(2):332-343; Kembro et al., 2014. Front Physiol 5:257).
Información de Series
Numerical integration of the ME-R model equations was performed with MatCont 2.4 in MATLAB 7.1, until steady-state solutions were obtained (i.e., when the magnitude of each time derivative was -10). Time series with duration of at least 6e6 ms were constructed by numerical integration of model equations. To allow transient states to vanish, the system was computed during a time lapse of 2 e8 ms.All studies were performed using the parameter setting optimized in our previous work (Kembro et al. 2013. Biophys J 104(2):332-343; Kembro et al., 2014. Front Physiol 5:257), with ADPm = 0.01mM, i.e. consistent with energized mitochondria under state 4 respiration. The two .txt file represents the time reference and the time series of variables output of the model, in order from left to right column: 1) Mitochondrial Ca+;2) Mitochondrial ADP; 3) Membrane potential; 4)Mitocondrial NADH; 5) Mitochondrial H+; 6) Mitochondrial Phosfate, Pi; 7) Isocitrate; 8) a-ketoglutarate; 9) Succinyl CoA; 10) Succinate; 11) Fumarate; 12) Malate; 13) Oxaloacetate; 14) NADPH; 15) Mitochondrial superoxide; 16) Extramitochondrial superoxide; 17) Mitochondrial hydrogen peroxide; 18) Extramitochondrial hydrogen peroxide; 19) Mitochondrial GSH; 20) Extramitochondrial GSH; 21)Mitochondrial GSSG; 22) Mitochondrial TrxSH2; 23) ExtramitochondrialTrxSH; 24)Mitochondrial PSSGm; 25) Extramitochondrial PSSG.
Información Técnica
Model-simulated time series were calculated with 0.013, 0.016, 0.0164, or 0.0216733 mM of SOD2, Shunt=0.04, SOD1 9.7 10-5 mM. External superoxide perturbation: amplitude=1 10-7 mM, period =30 s.
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CONICET_Digital_Nro.4bf0d45f-420e-475a-b3a5-451d0bdb2df6_A.txt
Series (variables) associated with SOD2 concentration 0.0216733mM
115.35 MB
CONICET_Digital_Nro.4bf0d45f-420e-475a-b3a5-451d0bdb2df6_D.txt
Series (variables) associated with SOD2 concentration 0.0164mM
35.77 MB
CONICET_Digital_Nro.4bf0d45f-420e-475a-b3a5-451d0bdb2df6_G.txt
Time associated with SOD2 concentration 0.016mM series
5.26 MB
CONICET_Digital_Nro.4bf0d45f-420e-475a-b3a5-451d0bdb2df6_J.txt
Time associated with SOD2 concentration 0.0164mM series
1.43 MB
CONICET_Digital_Nro.4bf0d45f-420e-475a-b3a5-451d0bdb2df6_M.txt
Time associated with SOD2 concentration 0.0216733 mM series
4.61 MB
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