Measurement of ascorbic acid and glutathione content in Cyanobacterium Synechocystis sp. PCC 6803
; Steelheart Molina, Maria Charlotte ; Alegre, Matias Leonel ; Berdun, Federico Juan ; Salerno, Graciela Lidia ; Bartoli, Carlos Guillermo ; Pagnussat, Gabriela Carolina ; Martin, María Victoria Otros responsables
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2020-10-20
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Standford University
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Bio-protocol
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2331-8325
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Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have precise and sensitive methods to determine the redox status in the cell and to detect variations in this antioxidants. In this protocol, we describe an improved method to estimate the content of both antioxidants (in their reduced and oxidized forms) from the same sample obtained from liquid cultures of Synechocystis sp. PCC 6803.
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Aguilera, Anabella; Steelheart Molina, Maria Charlotte; Alegre, Matias Leonel; Berdun, Federico Juan; Salerno, Graciela Lidia; et al.; Measurement of ascorbic acid and glutathione content in Cyanobacterium Synechocystis sp. PCC 6803; Standford University; Bio-protocol; 10; 20; 20-10-2020; 1-7
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