New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides

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dc.conicet.fuenteElsevier
dc.conicet.organizacionIstituto Per la Sintesi Organica E la Fotoreattivita, Bologna
dc.conicet.organizacionConsiglio Nazionale delle Ricerche
dc.conicet.organizacionConsiglio Nazionale delle Ricerche
dc.conicet.organizacionUniversidad de Buenos Aires. Facultad de Farmacia y Bioquímica
dc.conicet.organizacionConsejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay
dc.conicet.organizacionConsiglio Nazionale delle Ricerche
dc.conicet.organizacionConsiglio Nazionale delle Ricerche
dc.conicet.organizacionInstitute of Nuclear Chemistry and Technology
dc.conicet.organizacionInstitute of Nuclear Chemistry and Technology
dc.conicet.paisOrganizacionItalia
dc.conicet.paisOrganizacionItalia
dc.conicet.paisOrganizacionItalia
dc.conicet.paisOrganizacionArgentina
dc.conicet.paisOrganizacionArgentina
dc.conicet.paisOrganizacionItalia
dc.conicet.paisOrganizacionItalia
dc.conicet.paisOrganizacionPolonia
dc.conicet.paisOrganizacionPolonia
dc.conicet.produccionid8533297
dc.contributor.authorChatgilialoglu, Chryssostomos
dc.contributor.authorKrokidis, Marios G.
dc.contributor.authorMasi, Annalisa
dc.contributor.authorBarata Vallejo, Sebastian
dc.contributor.authorFerreri, Carla
dc.contributor.authorTerzidis, Michael A.
dc.contributor.authorSzreder, Tomasz
dc.contributor.authorBobrowski, Krzysztof
dc.date.accessioned2022-05-02T11:29:55Z
dc.date.available2022-05-02T11:29:55Z
dc.date.issued2019-10
dc.date.updated2022-04-26T17:08:19Z
dc.description.abstractThe reaction of hydroxyl radical (HO•) with DNA produces many primary reactive species and many lesions as final products. In this study, we have examined the optical spectra of intermediate species derived from the reaction of HO• with a variety of single- and double-stranded oligodeoxynucleotides and ct-DNA in the range of 1 µs to 1 ms by pulse radiolysis using an Intensified Charged Coupled Device (ICCD) camera. Moreover, we applied our published analytical protocol based on an LC-MS/MS system with isotopomeric internal standards to enable accurate and precise measurements of purine lesion formation. In particular, the simultaneous measurement of the four purine 50,8-cyclo-20-deoxynucleosides (cPu) and two 8-oxo-7,8-dihydro-20-deoxypurine (8-oxo-Pu) was obtained upon reaction of genetic material with HO• radicals generated either by γ-radiolysis or Fenton-type reactions. Our results contributed to the debate in the literature regarding absolute level of lesions, method of HO• radical generation, 50R/50S diastereomeric ratio in cPu, and relative abundance between cPu and 8-oxo-Pu.es
dc.description.filFil: Chatgilialoglu, Chryssostomos. Istituto Per la Sintesi Organica E la Fotoreattivita, Bologna; Italia
dc.description.filFil: Krokidis, Marios G.. Consiglio Nazionale delle Ricerche; Italia
dc.description.filFil: Masi, Annalisa. Consiglio Nazionale delle Ricerche; Italia
dc.description.filFil: Barata Vallejo, Sebastian. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina
dc.description.filFil: Ferreri, Carla. Consiglio Nazionale delle Ricerche; Italia
dc.description.filFil: Terzidis, Michael A.. Consiglio Nazionale delle Ricerche; Italia
dc.description.filFil: Szreder, Tomasz. Institute of Nuclear Chemistry and Technology; Polonia
dc.description.filFil: Bobrowski, Krzysztof. Institute of Nuclear Chemistry and Technology; Polonia
dc.formatapplication/pdfes
dc.identifier.citationChatgilialoglu, Chryssostomos; Krokidis, Marios G.; Masi, Annalisa; Barata Vallejo, Sebastian; Ferreri, Carla; et al.; New insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotides; Molecular Diversity Preservation International; Molecules; 24; 21; 10-2019; 1-20es
dc.identifier.institucionalCONICET
dc.identifier.issn1420-3049es
dc.identifier.repoCONICET Digital
dc.identifier.urihttp://hdl.handle.net/11336/156176
dc.journal.number21es
dc.journal.pagination1-20es
dc.journal.paisSuizaes
dc.journal.titleMoleculeses
dc.journal.volume24es
dc.language.isoenges
dc.publisherMolecular Diversity Preservation Internationales
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/24/21/3860
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/molecules24213860
dc.rightsinfo:eu-repo/semantics/openAccess*
dc.rights.urihttps://creativecommons.org/licenses/by/2.5/ar/*
dc.subject50,8-CYCLOPURINESes
dc.subject8-OXO-DGes
dc.subjectDNA DAMAGEes
dc.subjectFENTON REACTIONes
dc.subjectFREE RADICALSes
dc.subjectGAMMA RADIOLYSISes
dc.subjectOLIGONUCLEOTIDESes
dc.subjectPULSE RADIOLYSISes
dc.subject.classificationQuímica Orgánicaes
dc.subject.classificationCiencias Químicases
dc.subject.classificationCIENCIAS NATURALES Y EXACTASes
dc.titleNew insights into the reaction paths of hydroxyl radicals with purine moieties in DNA and double-stranded oligodeoxynucleotideses
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion

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