Neuronal entropy depends of the level of alertness: data
Otros responsables
Fecha
Publicador:
Consejo Nacional de Investigaciones Científicas y Técnicas
Fecha de depósito:
17/10/2023
Fecha de creación:
01/01/2013-31/12/2014
Clasificación temática
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Resumen
Microelectrode recordings (MER) obtained from adult rats weighing 250–350 g. assigned to the control group of an experimental Parkinson’s research protocol (Andres et al., 2014). The goal of the experiments was to record the spontaneous activity of the globus pallidus pars interna (GPi, previously called entopeduncular nucleus) under conditions of alertness, environmental silence and blindfoldednes.
Métodos
The neuronal recordings used for the present work were originally produced within an experimental Parkinson’s research protocol that followed the partial-lesion model originally described by Sauer and Oertel (1994). The signals analyzed correspond to microelectrode recordings (MER) obtained from adult rats weighing 250–350 g. assigned to the control group of the aforementioned protocol (Andres et al., 2014). The goal of the experiments was to record the spontaneous activity of the globus pallidus pars interna (GPi, previously called entopeduncular nucleus) under conditions of alertness, environmental silence and blindfoldedness. Optimal anesthetic and analgesic medication was used for the recording surgeries, with a three-drug profile consisting of a combination of tramadol, lidocaine and chloral hydrate. Following anesthesia, animals were placed in a restraining device built ad hoc with semi-rigid plastic and a high-quality thermal insulator on the inside. Once the animals were placed, fixed to the stereotactic frame and ready for surgery, we waited until they were completely awake, given the objective was to record spontaneous neuronal activity under complete alertness. The awakening process was monitored periodically with a standardized evaluation of the tail reflex. The purpose of the device was to minimize the animals’ discomfort during the surgery. During the whole procedure animals remained only loosely bound. If they didn’t relax but attempted to move, that was considered an endpoint and the recording surgery was terminated. During the whole surgical procedure, the eyes of the animals were covered and all surgeries were conducted in identical conditions of environmental silence. Recording coordinates fell within the limits defined as the GPi by the last edition of Paxinos and Watson’s (1997) Atlas. Neuronal recordings were obtained using glass- insulated platinum/iridium (Pt/Ir 80/20%) microelectrodes with nominal impedance of 0.8 – 1.2 megohms (mTSPBN-LX1, FHC Inc, Bowdoin, ME, USA). Signals were amplified, conditioned and monitored with an analog oscilloscope, digitized with a dedicated acquisition system (1401 plus, CED) and saved in a computer running Spike 2.0 software. The sampling rate was 20 kHz and total amplification including probe was ×10,000, checked with a built-in calibration signal of 1 mV p-p at the beginning of each experiment.
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