Actividad fumigante de aceites esenciales de Schinus molle (Anacardiaceae) y Tagetes terniflora (Asteraceae) sobre adultos de Pediculus humanus capitis (Insecta; Anoplura; Pediculidae)
; Werdin Gonzalez, Jorge Omar
; Benzi, Verónica Soledad
; Ferrero, Adriana Alicia Otros responsables
Fecha
04/2009
Editorial
Universidad de Santiago de Chile
Revista
Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas
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Institución organizadora
ISSN
0717-7917
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Español
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Resumen
Los aceites esenciales de hojas se aislaron por hidrodestilación. Los bioensayos se realizaron a 28 ± 2 ºC, 65% ± 5 H.R y en oscuridad. Se utilizaron cajas de Petri de vidrio (5cm de diámetro), en sus bases se colocaron 50 µL de los aceites puros. La caja se cubrió con una tapa plástica perforada que posee un tejido de hilo. Sobre este se liberaron 10 insectos protegidos por una segunda caja con diámetro equivalente. El conjunto de cajas se cerró con cinta adhesiva. Se realizaron tres réplicas por tratamiento. Como control se utilizaron cajas sin aceites. El porcentaje de volteo se evaluó cada 5 minutos durante una hora. Se calculó el TV50 por un análisis Probit. El TV50 fue 12,75 min para el aceite de Schinus molle y para el de Tagetes terniflora de 23,44 min. Ambos aceites podrían ser utilizados para el control de Pediculus humanus capitis.
Dried leaves of each species were hydrodistilled. The bioassays were performed at 28 ± 2 ºC, 65% ± 5 RH and in darkness. Each experimental unit consisted of a glass Petri dish (5cm diameter) containing 50 µL of the essentials oils on a micro coverglass. Then, it was covered with a lid with a fine wire sieve attached over the central hole. Batches of 10 adults were placed over the sieve. Each unit was then covered with another Petri dish. Control units were the same but without addition of any substance. All treatmentes wre replicated three times. The percentage of knockdown was evaluated every 5 min for one hour. The KT50 value was calculated by probit analysis. The results showed that the KT50 for Schinus molle was 12.75 min and for Tagetes terniflora was 23.44. Both essential oils could be considered for the control of Pediculus humanus capitis.
Dried leaves of each species were hydrodistilled. The bioassays were performed at 28 ± 2 ºC, 65% ± 5 RH and in darkness. Each experimental unit consisted of a glass Petri dish (5cm diameter) containing 50 µL of the essentials oils on a micro coverglass. Then, it was covered with a lid with a fine wire sieve attached over the central hole. Batches of 10 adults were placed over the sieve. Each unit was then covered with another Petri dish. Control units were the same but without addition of any substance. All treatmentes wre replicated three times. The percentage of knockdown was evaluated every 5 min for one hour. The KT50 value was calculated by probit analysis. The results showed that the KT50 for Schinus molle was 12.75 min and for Tagetes terniflora was 23.44. Both essential oils could be considered for the control of Pediculus humanus capitis.
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