Synergistic Effect of Dichloroacetate and Fluconazole on the Growth of Saccharomyces cerevisiae

Abstract

The development of resistance to antifungals is a relevant issue during the treatment of infections. Using S. cerevisiae cells, we studied the effect of dichloroacetate (DCA), onthe growth of the microorganism in the presence of fluconazole (FCZ). The results obtained show that DCA (2.0 - 3.0 mg/ml), sensitizes yeast cells to growth inhibition by fluconazole.Results indicate that the external pH reduction produced by DCA decreases the electrochemical proton gradient necessary for the extrusion of toxic compounds to yeastmetabolism. This effect favors a synergism strategy leading to lower drug doses and reducing drugs side effects.