Analysing the solution state of protein structurem interactions and ligands by spectroscopic methods

Abstract

This chapter provides a general overview of several spectroscopic techniques currently available for studying protein secondary structure in solution and assessing its changes in response to ligand and/or environmental interaction. These techniques include ultraviolet—visible (UV—vis) absorption spectroscopy, circular dichroism (CD) spectroscopy, fluorescence spectroscopy, Fourier transform infrared (FT-IR) and Raman spectroscopy, and photon correlation spectroscopy.