Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement

Volver a la Vista simple
dc.conicet.organizacionMahidol University. Faculty of Sciences
dc.conicet.organizacionConsejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía
dc.conicet.organizacionUniversidad de Buenos Aires
dc.conicet.organizacionMahidol University. Faculty of Science
dc.conicet.organizacionMahidol University. Faculty of Science
dc.conicet.organizacionNational Science and Technology Development Agency
dc.conicet.organizacionMahidol University. Faculty of Science
dc.conicet.organizacionMahidol University. Faculty of Science
dc.conicet.paisOrganizacionTailandia
dc.conicet.paisOrganizacionArgentina
dc.conicet.paisOrganizacionArgentina
dc.conicet.paisOrganizacionTailandia
dc.conicet.paisOrganizacionTailandia
dc.conicet.paisOrganizacionTailandia
dc.conicet.paisOrganizacionTailandia
dc.conicet.paisOrganizacionTailandia
dc.conicet.produccionid2783505
dc.contributor.authorLeasen, Suthisa
dc.contributor.authorHodak, Jose Hector
dc.contributor.author Srisala, Jiraporn
dc.contributor.authorSrikhirin, Toemsak
dc.contributor.authorSritunyalucksana, Kallaya
dc.contributor.authorVeerasai, Waret
dc.contributor.authorDangtip, Somsak
dc.date.accessioned2016-11-25T17:26:05Z
dc.date.available2016-11-25T17:26:05Z
dc.date.issued2013-09
dc.date.updated2016-10-25T19:27:38Z
dc.description.abstractSelf-assembled monolayers (SAM) of 3-mercaptopropionic acid (MPA) were applied on tin-doped indium oxide (ITO) surfaces and used as a working electrode for sensing DNA hybridization. The concentration of probe single stranded DNA (ssDNA), complemented with target DNA, was optimized for the highest yield immobilization on MPA/ITO platform. The ssDNA/MPA/ITO was allowed to hybridize to target DNA prepared from PCR amplification that first tested by the synthesized complementary sequences. Both cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed for investigating probe ssDNA immobilization and target DNA hybridization. For fast and low concentration detecting purposes, methylene blue (MB) coupled with differential pulse voltammetry (DPV) was used for detecting the target DNA hybridization events.es
dc.description.filFil: Leasen, Suthisa . Mahidol University. Faculty of Sciences; Tailandia
dc.description.filFil: Hodak, Jose Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires; Argentina
dc.description.filFil: Srisala, Jiraporn. Mahidol University. Faculty of Science; Tailandia
dc.description.filFil: Srikhirin, Toemsak . Mahidol University. Faculty of Science; Tailandia
dc.description.filFil: Sritunyalucksana, Kallaya . National Science and Technology Development Agency; Tailandia
dc.description.filFil: Veerasai, Waret . Mahidol University. Faculty of Science; Tailandia
dc.description.filFil: Dangtip, Somsak . Mahidol University. Faculty of Science; Tailandia
dc.formatapplication/pdfes
dc.identifier.citationLeasen, Suthisa ; Hodak, Jose Hector; Srisala, Jiraporn; Srikhirin, Toemsak ; Sritunyalucksana, Kallaya ; et al.; Detecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurement; Scientific.Net; Advanced Materials Research; 770; 9-2013; 402-408es
dc.identifier.issn1662-8985
dc.identifier.urihttp://hdl.handle.net/11336/8401
dc.journal.ciudadDurnten-Zurich, Switzerlandes
dc.journal.pagination402-408es
dc.journal.paisSuizaes
dc.journal.titleAdvanced Materials Researches
dc.journal.volume770es
dc.language.isoenges
dc.publisherScientific.Netes
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/url/http://www.scientific.net.sci-hub.org/AMR.770.402
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4028/www.scientific.net/AMR.770.402
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/hdl/http://ir.sc.mahidol.ac.th/handle/123456789/76
dc.rightsinfo:eu-repo/semantics/openAccess*
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/es
dc.subjectHybridizationes
dc.subjectTin-Doped Indium Oxidees
dc.subject3-Mercaptopropionic Acides
dc.subjectDifferential Pulse Voltammetryes
dc.subjectMethylene Bluees
dc.subject.classificationFísico-Química, Ciencia de los Polímeros, Electroquímicaes
dc.subject.classificationCiencias Químicases
dc.subject.classificationCIENCIAS NATURALES Y EXACTASes
dc.subject.classificationQuímica Analíticaes
dc.subject.classificationCiencias Químicases
dc.subject.classificationCIENCIAS NATURALES Y EXACTASes
dc.titleDetecting DNA-DNA hybridization at 3-mercaptopropionic acid self-assembled on tin-doped indium oxide film with electrochemical measurementes
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion

Archivos

Bloque original

Miniatura
Nombre:
CONICET_Digital_Nro.10345.pdf
Tamaño:
306.71 KB
Formato:
Adobe Portable Document Format
Descargar

Colecciones

Articulos(INQUIMAE)
Volver a la Vista simple