Comparative and integrative omic analysis focused on chaperones and interactors in a cultivated and an exotic tomato at different fruit ripening stages

Abstract

Heat Shock Proteins (HSPs) are a superfamily of chaperones that have been characterized in different organisms. In plants, HSPs promote protein folding and deaggregation during abiotic stress or developmental changes. The aim of this work was to integrate several omic-data to identify chaperone and putative interactors in Solanum lycopersicum domesticated cultivar Caimanta (C) and in the latinoamerican wild Solanum pimpinellifolium (P) genotypes during fruit ripening (FR), which are the parental lines of different breeding populations obtained by our research group. We were able to identify newly putative interactors and simultaneously induced HSP members at the transcription and proteomic levels. This integrative approach also revealed gene/protein families related to chlorophyll content, photosynthesis and HSP70 chaperones in C. Furthermore, P was enriched with chaperones, including HSP20, ATPase families, (characteristic of HSP90 and HSP100) and other protein families involved in oxidoreductase activity, supporting the hypothesis of the existence of a relationship between HSPs and developmental processes as FR. Finally, we found that some of these up-regulated chaperones show the presence of heat shock element motifs in their promoters. Proteomic coupled with transcriptomics and interactomics facilitated the exploration of a good new gene-context at the tomato development.