Promising co-inoculation strategies to reduce arsenic toxicity in soybean

Abstract

Arsenic (As) is cause for concern worldwide due to its high toxicity. Its presence in agricultural soils and groundwater adversely affects soybean (Glycine max L.) growth and yield, and also endangers food safety. Plant growth-promoting rhizobacteria (PGPR) could be used as part of cost-effective and eco-friendly strategies to mitigate As phytotoxicity. However, simple inoculation of soybean with PGPR Bradyrhizobium japonicum E109 (E109), a common practice in Argentina, is not effective in counteracting the effects of As exposure. Our aim was to assess whether the response of soybean to arsenate (AsV) and arsenite (AsIII) could be helpfully modulated by co-inoculating E109 with the free-living PGPRs Azospirillum brasilense Cd (Cd) or Bacillus pumilus SF5 (SF5). Co-inoculation with E109+SF5 alleviated As-induced depletion of chlorophyll a and b, and carotenoid content, reaching an increase of 26, 28 y 31%, respectively. It also enhanced nodulation (15-19%) under As exposure. E109+Cd and E109+SF5 induced changes in the antioxidant system, which could be related to the maintenance of redox homeostasis. Moreover, As accumulation was reduced by 53% in aerial parts of plants inoculated with E109+Cd, and by 16% in the roots of those inoculated with E109+SF5. The strains selected show interesting potential for the development of biotechnological schemes to improve soybean yield while guaranteeing safer food production.