From Enzyme Encapsulation to Environmental Solutions: Photostable Laccase in Dopc Vesicles

Abstract

Our results demonstrate that DOPC vesicles effectively encapsulate the laccase enzyme, maintaining its activity within the vesicles. Moreover, the substrate syringaldazine, a modelcompound for laccase activity studies, was able to permeate through the bilayer. Notably, encapsulated laccase exhibited significantly higher retention of enzymatic activity (REA) postirradiation compared to the free enzyme. In conclusion, both unilamellar and multilamellar vesicles provide substantial photoprotection for laccase against UVB light. This protectiveeffect is attributed to the enzyme’s confinement within a system that scatters high-energy photons. Furthermore, the simplicity and ease of large-scale production of multilamellar vesicles present an advantageous option for various applications, including bioremediation.