Drug repurposing: tolfenamic acid inactivates PrbP, a transcriptional accessory protein in liberibacter asiaticus

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dc.conicet.organizacionUniversity of Florida
dc.conicet.organizacionUniversity of Florida
dc.conicet.organizacionUniversity of Florida
dc.conicet.organizacionUniversity of Florida
dc.conicet.organizacionUniversity of Florida
dc.conicet.organizacionConsejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos
dc.conicet.organizacionUniversity of Florida
dc.conicet.organizacionUniversity of Florida
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.paisOrganizacionArgentina
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.paisOrganizacionEstados Unidos
dc.conicet.produccionid6352543
dc.contributor.authorGardner, Christopher L.
dc.contributor.authorPagliai, Fernando A.
dc.contributor.authorPan, Lei
dc.contributor.authorBojilova, Lora
dc.contributor.authorTorino, Maria Ines
dc.contributor.authorLorca, Graciela L.
dc.contributor.authorGonzalez, Claudio F.
dc.date.accessioned2017-11-29T20:05:28Z
dc.date.available2017-11-29T20:05:28Z
dc.date.issued2016-10-18
dc.date.updated2017-07-13T18:58:42Z
dc.description.abstractCLIBASIA_01510, PrbP, is a predicted RNA polymerase binding protein in Liberibacter asiaticus. PrbP was found to regulate expression of a small subset of ribosomal genes through interactions with the β-subunit of the RNA polymerase and a short, specific sequence on the promoter region. Molecular screening assays were performed to identify small molecules that interact with PrbP in vitro. Chemical hits were analyzed for therapeutic efficacy against L. asiaticus via an infected leaf assay, where the transcriptional activity of L. asiaticus was found to decrease significantly after exposure to tolfenamic acid. Similarly, tolfenamic acid was found to inhibit L. asiaticus infection in highly symptomatic citrus seedlings. Our results indicate that PrbP is an important transcriptional regulator for survival of L. asiaticus in planta, and the chemicals identified by molecular screening assays could be used as a therapeutic treatment for huanglongbing disease.es
dc.description.filFil: Gardner, Christopher L.. University of Florida; Estados Unidos
dc.description.filFil: Pagliai, Fernando A.. University of Florida; Estados Unidos
dc.description.filFil: Pan, Lei. University of Florida; Estados Unidos
dc.description.filFil: Bojilova, Lora. University of Florida; Estados Unidos
dc.description.filFil: Torino, Maria Ines. University of Florida; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.filFil: Lorca, Graciela L.. University of Florida; Estados Unidos
dc.description.filFil: Gonzalez, Claudio F.. University of Florida; Estados Unidos
dc.formatapplication/pdfes
dc.identifier.citationGardner, Christopher L.; Pagliai, Fernando A.; Pan, Lei; Bojilova, Lora; Torino, Maria Ines; et al.; Drug repurposing: tolfenamic acid inactivates PrbP, a transcriptional accessory protein in liberibacter asiaticus; Frontiers; Frontiers in Microbiology; 7; 18-10-2016; 1-16;1630-1630es
dc.identifier.eissn1664-302Xes
dc.identifier.institucionalCONICET
dc.identifier.issn1664-302Xes
dc.identifier.repoCONICET Digital
dc.identifier.urihttp://hdl.handle.net/11336/29307
dc.journal.ciudadLausannees
dc.journal.pagination1-16;1630-1630es
dc.journal.paisSuizaes
dc.journal.titleFrontiers in Microbiologyes
dc.journal.volume7es
dc.language.isoenges
dc.publisherFrontierses
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fmicb.2016.01630
dc.relation.alternativeidinfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2016.01630/full
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/*
dc.subjectTranscriptional Accessory Proteines
dc.subjectLiberibacter Asiaticuses
dc.subjectTolfenamic Acides
dc.subjectAntimicrobiales
dc.subjectCitruses
dc.subject.classificationOtras Ciencias Biológicases
dc.subject.classificationCiencias Biológicases
dc.subject.classificationCIENCIAS NATURALES Y EXACTASes
dc.titleDrug repurposing: tolfenamic acid inactivates PrbP, a transcriptional accessory protein in liberibacter asiaticuses
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion

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