Screening of tolerance of Atriplex vulgatissima under zinc or lead experimental conditions. An integrative perspective by using the integrated biological response index (IBRv2)

Abstract

The search for plants with a high capacity to tolerate and accumulate metals is an important issue in phytoremediation. In this sense, this study was conducted in the halophyte Atriplex vulgatissima to evaluate the effects of different concentrations of lead (Pb, 50 and 100 μM) or zinc (Zn, 100 and 200 μM) on morphological, physiological, and biochemical parameters as well as the accumulation patterns of this species. The results indicated that while essential metal Zn showed high translocation from roots to shoots (TF > 1), non-essential Pb was mainly accumulated in the roots (BCF>1). Regarding shape, both metals induced slenderness of the blade, but only Zn treatment reduced leaf size. No difference in biomass production and photosynthetic parameters was found between Pb and Zn treatments. Pb treatments did not show significant differences between treatments regarding water content (WC), pigment concentration, and the activity of superoxide dismutase (SOD) and guaiacol peroxidase (GPx), but did result in a decrease in catalase activity at 100 μM Pb. On the other hand, 200 μM Zn leads to a clear reduction in WC and pigment concentrations, along with an increase in SOD and GPx activities. In addition, ascorbate peroxidase (APx) activity showed a hormesis effect at 50 μM Pb and 100 μM Zn. Malondialdehyde increased with both Pb and Zn treatments. The integrated biological index (IBRv2) indicated that 200 μM Zn was the most affected treatment (IBRv2 = 19.02) and that under the same concentrations of metals (100 μM Pb or Zn), Pb treatments presented major stress (IBRv2 = 11.55). A. vulgatissima is a metallophyte with the potential for Pb phytostabilization and Zn phytoextraction, as well as a bioindicator of these metals. Its high biomass and deep roots, combined with its halophytic traits, make it suitable for bioremediation and monitoring programs.