Functional postnatal maturation of the medial olivocochlear efferent-outer hair cell synapse

Abstract

The organ of Corti, the auditory mammalian sensory epithelium, contains two types of mechanotransducer cells, inner and outer hair cells (IHCs and OHCs, respectively). IHCs are involved in conveying acoustic stimuli to the central nervous system while OHCs are implicated in the fine tuning and amplification of sounds. OHCs are innervated by medial olivocochlear (MOC) cholinergic efferent fibers. The functional characteristics of the MOC-OHC synapse during maturation were assessed by electrophysiological and pharmacological methods in mouse organs of Corti at postnatal days (P)11-13, hearing onset in altricial rodents, and at P20-22 when the OHCs are morphologically and functionally mature. Synaptic currents were recorded in whole-cell voltage-clamped OHCs while electrically stimulating the MOC fibers. A progressive increase in the number of functional MOC-OHC synapses, as well as in their strength and efficacy was observed between P11-13 and P20-22. At hearing onset, the MOC-OHC synapse presented facilitation upon MOC fibers high-frequency stimulation which disappeared at mature stages. In addition, important changes were found in the VGCC that are coupled to transmitter release. Ca2+ flowing in through L-type VGCCs contribute to trigger ACh release together with P/Q- and R-type VGCCs at P11-13 but not at P20-22. Interestingly, N-type VGCCs were found to be involved in this process at P20-22 but not at hearing onset. Moreover, the degree of compartmentalization of calcium channels with respect to BK channels and presynaptic release components significantly increased from P11-13 to P20-22. These results suggest that the MOC-OHC synapse is immature at the onset of hearing.