Simultaneous Quantification of Curcumin and Ascorbic Acid in Tablets: A Student-Led HPLC and UV–Vis Laboratory Experiment

Abstract

High-performance liquid chromatography (HPLC) and UV–vis spectrophotometry are indispensable techniques in pharmaceutical and food analysis, yet students often struggle to connect theoretical principles with real-world applications. This laboratory experiment addresses this gap by engaging third-year chemistry students in the optimization and validation of HPLC and UV–vis methods for the simultaneous quantification of curcumin and ascorbic acid in commercial tablets. Designed as a capstone project for a Chemical Technician program, the activity emphasizes inquiry-based learning: student teams design protocols, prepare calibration standards, extract analytes from complex matrices, and validate their methods by calculating detection limits (LODs), quantification limits (LOQs), recovery rates, and linear dynamic ranges. The project’s authenticity─using industrially relevant samples and quality control metrics─proved pedagogically powerful: postlab surveys revealed enhanced student confidence in troubleshooting and method development. By integrating collaborative problem-solving, regulatory-compliant documentation, and comparative analysis of two analytical techniques, this experiment bridges classroom theory with professional practice. The modular design allows adaptation for courses with varying resources, and all supporting materials (pre/post assessments, instructor guides, and sample data sets) are provided for implementation.