Capacidad emulsionante de sueros de soja

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Fecha
04/2010
Editorial
Laboratorio Tecnológico del Uruguay
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Innotec
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1688-6607
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Español
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Resumen
El objetivo fue analizar la capacidad emulsionante de diferentes sueros de soja como productos integrales. Se ensayaron: suero de soja liofilizado (SSL), suero de soja liofilizado y calentado (SSLC), suero de tofu liofilizado (STL) y suero de tofu secado térmicamente (STST). Las emulsiones fueron realizadas a igual nivel de proteína bruta (0,1–1,0% p/v, buffer fosfato 10 mm, Φmásico =0,33, Ultraturrax T-25). La estabilidad de las emulsiones (medida a través del aceite separado) decreció en el orden: SSLC > STST > STL > SSL. Estos resultados se correlacionan con el índice de actividad emulsificante con y sin SDS. Por medidas de BackScattering y difracción láser se observó una reducción del tamaño de partícula en las emulsiones preparadas con sueros calentados (SSLC respecto a SSL y STST respecto a STL). Se observaron diferencias en la capacidad emulsionante de SSLC y STST, atribuidas a las condiciones de calentamiento. Las reacciones de desnaturalización y de formación de agregados glicosilados en el suero de soja liofilizado y calentado reducirían la formación de flóculos en la emulsión, principal mecanismo de desestabilización en estas emulsiones.
The objective was to analyze the emulsifying capacity of different soybean wheys as integral products. The following samples were tested: lyophilized soybean whey (LSW), heated LSW (HLSW), lyophilized tofu whey (LTW) and heat-dried tofu whey (HDTW). The emulsions were carried out at same level of total protein (0.1–1.0% w/v, phosphate buffer 10 mm, Φw=0.33, Ultraturrax T-25). Emulsion stability (measured by the separated oil) decreased in the order: HLSW> HDTW> LTW> LSW. These results are correlated with emulsifying activity index (EAI) with and without SDS. Assays of backscattering and laser diffraction showed a reduction of particle size in emulsions prepared with heated whey (HLSW and HDTW in comparison with LSW and LTW, respectively). The differences between HLSW and TDTW can be attributed to the glycosilated products generated during thermal treatment under anhydrous conditions, factors which prevent the flock formation postulated as the main mechanism to destabilize these emulsions.
The objective was to analyze the emulsifying capacity of different soybean wheys as integral products. The following samples were tested: lyophilized soybean whey (LSW), heated LSW (HLSW), lyophilized tofu whey (LTW) and heat-dried tofu whey (HDTW). The emulsions were carried out at same level of total protein (0.1–1.0% w/v, phosphate buffer 10 mm, Φw=0.33, Ultraturrax T-25). Emulsion stability (measured by the separated oil) decreased in the order: HLSW> HDTW> LTW> LSW. These results are correlated with emulsifying activity index (EAI) with and without SDS. Assays of backscattering and laser diffraction showed a reduction of particle size in emulsions prepared with heated whey (HLSW and HDTW in comparison with LSW and LTW, respectively). The differences between HLSW and TDTW can be attributed to the glycosilated products generated during thermal treatment under anhydrous conditions, factors which prevent the flock formation postulated as the main mechanism to destabilize these emulsions.
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