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dc.contributor.author
Grunhut, Marcos
dc.contributor.author
Centurión, María Eugenia
dc.contributor.author
Fernández Band, Beatriz Susana
dc.date.available
2020-01-20T21:56:08Z
dc.date.issued
2007-01
dc.identifier.citation
Grunhut, Marcos; Centurión, María Eugenia; Fernández Band, Beatriz Susana; PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique; Taylor & Francis; Analytical Letters; 40; 10; 1-2007; 2016-2031
dc.identifier.issn
0003-2719
dc.identifier.uri
http://hdl.handle.net/11336/95338
dc.description.abstract
An enzymatic stopped-flow-injection analysis is proposed for simultaneous determination of levodopa and carbidopa in pharmaceutical preparations. The dopaquinones obtained after the oxidation catalized by the enzyme were measured by spectrophotometric method. A reduced calibration matrix based on a central composite experimental design was built and Partial Least Squares (PLS) was applied on the spectral data after reaction with the enzyme. The LOD was 0.015 and 0.0028 mg ml-1, respectively and the sample throughput was 22.5 h-1. The proposed method was applied to pharmaceutical preparations and the results are in close agreement with pharmacopeial method. The recovery study and results were satisfactory.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CARBIDOPA
dc.subject
LEVODOPA
dc.subject
PLS
dc.subject
POLYPHENOL OXIDASE
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STOPPED-FLOW FIA
dc.subject.classification
Química Analítica
dc.subject.classification
Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
PLS regression in the spectrophotometric data for the simultaneous determination of levodopa and carbidopa in pharmaceutical preparations by using an enzymatic stopped-flow FIA technique
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-11-04T15:25:41Z
dc.journal.volume
40
dc.journal.number
10
dc.journal.pagination
2016-2031
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Londres
dc.description.fil
Fil: Grunhut, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
dc.description.fil
Fil: Centurión, María Eugenia. Universidad Nacional del Sur. Departamento de Química; Argentina
dc.description.fil
Fil: Fernández Band, Beatriz Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
dc.journal.title
Analytical Letters
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/00032710701486249
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/00032710701486249
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