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dc.contributor.author
Vasconsuelo, Andrea Anahi
dc.contributor.author
Milanesi, Lorena Magdalena
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Boland, Ricardo Leopoldo
dc.date.available
2020-01-09T20:48:56Z
dc.date.issued
2010-03
dc.identifier.citation
Vasconsuelo, Andrea Anahi; Milanesi, Lorena Magdalena; Boland, Ricardo Leopoldo; Participation of HSP27 in the antiapoptotic action of 17β-estradiol in skeletal muscle cells; Springer; Cell Stress & Chaperones; 15; 2; 3-2010; 183-192
dc.identifier.issn
1355-8145
dc.identifier.uri
http://hdl.handle.net/11336/94249
dc.description.abstract
Exposure to 17β-estradiol prior to induction of apoptosis protects skeletal muscle cells against damage. The mechanism involved in this protective action of the hormone is poorly understood. In the present study, using the murine muscle cell line C2C12, evidence was obtained that inhibition of H 2O2-induced apoptosis by the estrogen requires the participation of heat shock protein 27 (HSP27). Reverse transcriptase polymerase chain reaction, Western blot, and immunocytochemistry assays showed that 17βestradiol induces a time-dependent (5-60 min) increase in the expression of HSP27. In addition, in presence of quercetin, an inhibitor of HSPs, the antiapoptotic effect of the hormone was diminished. More specifically, blockage experiments with short interference RNA targeting HSP27 confirmed the role of this chaperone in the protective effect of the steroid. 17β-Estradiol abolished caspase-3 cleavage elicited by H2O2. Coimmunoprecipitation assays suggested physical interaction of HSP27 with caspase-3 in presence of estradiol. Furthermore, we observed that this chaperone interacts with estrogen receptors (ER) β in mitochondria. Then, this study suggests that HSP27 plays a new role in the antiapoptotic action triggered by 17β-estradiol by modulating caspase-3 activity and stabilizing ERβ in skeletal muscle cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
17Β-ESTRADIOL
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APOPTOSIS
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CASPASE-3
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ERΒ
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HSP27
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SKELETAL MUSCLE
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Participation of HSP27 in the antiapoptotic action of 17β-estradiol in skeletal muscle cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-11-25T18:37:49Z
dc.identifier.eissn
1466-1268
dc.journal.volume
15
dc.journal.number
2
dc.journal.pagination
183-192
dc.journal.pais
Alemania
dc.description.fil
Fil: Vasconsuelo, Andrea Anahi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
dc.description.fil
Fil: Milanesi, Lorena Magdalena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
dc.description.fil
Fil: Boland, Ricardo Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
dc.journal.title
Cell Stress & Chaperones
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12192-009-0132-y
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s12192-009-0132-y
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