Artículo
Participation of HSP27 in the antiapoptotic action of 17β-estradiol in skeletal muscle cells
Fecha de publicación:
03/2010
Editorial:
Springer
Revista:
Cell Stress & Chaperones
ISSN:
1355-8145
e-ISSN:
1466-1268
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Exposure to 17β-estradiol prior to induction of apoptosis protects skeletal muscle cells against damage. The mechanism involved in this protective action of the hormone is poorly understood. In the present study, using the murine muscle cell line C2C12, evidence was obtained that inhibition of H 2O2-induced apoptosis by the estrogen requires the participation of heat shock protein 27 (HSP27). Reverse transcriptase polymerase chain reaction, Western blot, and immunocytochemistry assays showed that 17βestradiol induces a time-dependent (5-60 min) increase in the expression of HSP27. In addition, in presence of quercetin, an inhibitor of HSPs, the antiapoptotic effect of the hormone was diminished. More specifically, blockage experiments with short interference RNA targeting HSP27 confirmed the role of this chaperone in the protective effect of the steroid. 17β-Estradiol abolished caspase-3 cleavage elicited by H2O2. Coimmunoprecipitation assays suggested physical interaction of HSP27 with caspase-3 in presence of estradiol. Furthermore, we observed that this chaperone interacts with estrogen receptors (ER) β in mitochondria. Then, this study suggests that HSP27 plays a new role in the antiapoptotic action triggered by 17β-estradiol by modulating caspase-3 activity and stabilizing ERβ in skeletal muscle cells.
Palabras clave:
17Β-ESTRADIOL
,
APOPTOSIS
,
CASPASE-3
,
ERΒ
,
HSP27
,
SKELETAL MUSCLE
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - BAHIA BLANCA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - BAHIA BLANCA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - BAHIA BLANCA
Citación
Vasconsuelo, Andrea Anahi; Milanesi, Lorena Magdalena; Boland, Ricardo Leopoldo; Participation of HSP27 in the antiapoptotic action of 17β-estradiol in skeletal muscle cells; Springer; Cell Stress & Chaperones; 15; 2; 3-2010; 183-192
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