Artículo
Influenza A virus negative strand RNA is translated for CD8+ T cell immunosurveillance
Hickman, Heather D.; Mays, Jacqueline W.; Gibbs, James; Kosik, Ivan; Magadan, Javier Guillermo
; Takeda, Kazuyo; Das, Suman; Reynoso, Glennys V.; Ngudiankama, Barbara F.; Wei, Jiajie; Shannon, John P.; McManus, Daniel; Yewdell, Jonathan W.
Fecha de publicación:
08/2018
Editorial:
American Association of Immunologists
Revista:
Journal of Immunology
ISSN:
0022-1767
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Probing the limits of CD8+ T cell immunosurveillance, we inserted the SIINFEKL peptide into influenza A virus (IAV)-negative strand gene segments. Although IAV genomic RNA is considered noncoding, there is a conserved, relatively long open reading frame present in segment 8, encoding a potential protein termed NEG8. The biosynthesis of NEG8 from IAV has yet to be demonstrated. Although we failed to detect NEG8 protein expression in IAV-infected mouse cells, cell surface Kb-SIINFEKL complexes are generated when SIINFEKL is genetically appended to the predicted C terminus of NEG8, as shown by activation of OT-I T cells in vitro and in vivo. Moreover, recombinant IAV encoding of SIINFEKL embedded in the negative strand of the neuraminidase-stalk coding sequence also activates OT-I T cells in mice. Together, our findings demonstrate both the translation of sequences on the negative strand of a single-stranded RNAvirus and its relevance in antiviral immunosurveillance.
Palabras clave:
INFLUENZA A
,
NEG8
,
IMMUNOSURVEILLANCE
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Articulos(IHEM)
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Citación
Hickman, Heather D.; Mays, Jacqueline W.; Gibbs, James; Kosik, Ivan; Magadan, Javier Guillermo; et al.; Influenza A virus negative strand RNA is translated for CD8+ T cell immunosurveillance; American Association of Immunologists; Journal of Immunology; 201; 4; 8-2018; 1222-1228
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