Artículo
Stability of the Salmonella Typhimurium rcsC11 mutant under different stress conditions
Torres, Mariela Analía
; Leccese Terraf, Maria Cecilia
; Minahk, Carlos Javier
; Delgado, Monica Alejandra
Fecha de publicación:
11/2019
Editorial:
Society for General Microbiology
Revista:
Microbiology-UK
ISSN:
1350-0872
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The virulence genes of Salmonella are modulated during infection by several regulatory systems, and the RcsCDB system is one of the most important of these. The S. Typhimurium EG14873 (rcsC11) strain harbours the rcsC11 point mutation, displaying a constitutive activation of this system, which is characterized by mucoid colonies and attenuated virulence phenotypes. In this work, the stability of the rcsC11 mutation was analysed under stress conditions. Under acid and anaerobic stresses, we observed the appearance of small and non-mucoid colonies of the rcsC11 strain. The sequencing of the rcsC gene from these colonies showed that the mutation is conserved. Moreover, we found that small colonies were also generated when the wild-type strain grew in acid and anaerobic conditions. It is worth noting that the transition from normal to atypical colonies of both strains only took place after several days of incubation and was not observed during eukaryotic cell infection. Therefore, the appearance of these atypical colonies is a characteristic feature of S. Typhimurium strains under stressful situations and does not involve a reversion of the rcsC11 allele and nor does it imply any risk to mammalian cells. Therefore, we propose that the S. Typhimurium rcsC11 strain is a good candidate for the development of attenuated vaccines.
Palabras clave:
SALMONELLA
,
MUTANT
,
INFECTION
,
VACCINE
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Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Torres, Mariela Analía; Leccese Terraf, Maria Cecilia; Minahk, Carlos Javier; Delgado, Monica Alejandra; Stability of the Salmonella Typhimurium rcsC11 mutant under different stress conditions; Society for General Microbiology; Microbiology-UK; 11-2019
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