Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture

Abstract

Toxigenic Aspergillus flavus and A. parasiticus fungal strains can contaminate a wide variety of food crops with the subsequent production of aflatoxins (AFs) resulting in severe economic losses and public health issues. Biological control is a promising approach to manage AFs contamination in pre- and post-harvested crops. In the present study, the effect of soil-borne Bacillus spp. strains on aflatoxigenic A. parasiticus growth and AFs production was evaluated and the culture supernatant of the most effective strain was evaluated for the presence of antifungal lipopeptides. Six Bacillus spp. strains were able to reduce A. parasiticus growth rate significantly (p < 0.05). Bacillus spp. RC1A was able to inhibit fungal growth almost completely, reducing growth rate to 0.16 mm/h and increasing Lag phase duration (31.72 h) (p < 0.0001). RC1A could also reduce AFB1 concentration produced by A. parasiticus (p < 0.0001). Organic solvent extraction and chromatographic analysis of RC1A culture supernatant showed the presence of bands corresponding to three of the main groups of lipopeptides (surfactin, iturin A and fengycin) at the expected retention factor (Rf) values; they were also confirmed by MALDI-MS analysis. These fractions were able to inhibit A. parasiticus growth and AFB1 production to non-detectable levels when tested separately in liquid culture media. The further study of the antifungal compounds produced by these strains will determine their potential use to manage AFs contamination in crops and feeds.